X-ray diffraction
2.24Å resolution

BtGH84 structure in complex with PUGNAc


Function and Biology Details

Reaction catalysed:
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
O-GlcNAcase BT_4395 Chain: A
Molecule details ›
Chain: A
Length: 737 amino acids
Theoretical weight: 84.59 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
  • Canonical: Q89ZI2 (Residues: 1-737; Coverage: 100%)
Gene name: BT_4395
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 185.64Å b: 51.34Å c: 81.46Å
α: 90° β: 96.97° γ: 90°
R R work R free
0.205 0.203 0.243
Expression system: Escherichia coli BL21