› 2vq2

X-ray diffraction
1.54Å resolution

Crystal structure of PilW, widely conserved type IV pilus biogenesis factor

Released:
DOI: 10.2210/pdb2vq2/pdb
PDB entry 2vq2 coloured by chain, front view.
PDB entry 2vq2 coloured by chain, side view.
PDB entry 2vq2 coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Structure of a widely conserved type IV pilus biogenesis factor that affects the stability of secretin multimers.
Trindade MB, Job V, Contreras-Martel C, Pelicic V, Dessen A
J Mol Biol 378 1031-9 (2008)
PMID: 18433773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fimbrial biogenesis and twitching motility protein Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.38 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JZ41 (Residues: 29-253; Coverage: 89%)
Gene name: NMB1309
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

5 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
Ligand CL 3 x CL
Ligand SO3 1 x SO3
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P6222
Unit cell:
a: 87.446Å b: 87.446Å c: 117.102Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
6 more

8 mentions without citation

Secretins: dynamic channels for protein transport across membranes.
Korotkov et al. (2011)
7 more