2vpd

X-ray diffraction
2.77Å resolution

Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pygopus homolog 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 67 amino acids
Theoretical weight: 7.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Y3Y4 (Residues: 333-398; Coverage: 16%)
Gene name: PYGO1
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
B-cell CLL/lymphoma 9 protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 35 amino acids
Theoretical weight: 3.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O00512 (Residues: 174-205; Coverage: 2%)
Gene name: BCL9

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 88.72Å b: 100.89Å c: 64.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.267
Expression system: Escherichia coli BL21