PDBe 2vlq

X-ray diffraction
1.6Å resolution

F86A mutant of E9 DNase domain in complex with Im9

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E9 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 36.058Å b: 77.49Å c: 41.807Å
α: 90° β: 100.21° γ: 90°
R-values:
R R work R free
0.166 0.164 0.199
Expression system: Escherichia coli