PDBe 2vje

X-ray diffraction
2.2Å resolution

Crystal Structure of the MDM2-MDMX RING Domain Heterodimer

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Mdm2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 64 amino acids
Theoretical weight: 7.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00987 (Residues: 428-491; Coverage: 13%)
Gene name: MDM2
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Protein Mdm4 Chains: B, D
Molecule details ›
Chains: B, D
Length: 63 amino acids
Theoretical weight: 7.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15151 (Residues: 428-490; Coverage: 13%)
Gene names: MDM4, MDMX
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 54.562Å b: 40.769Å c: 77.514Å
α: 90° β: 109.06° γ: 90°
R-values:
R R work R free
0.198 0.196 0.223
Expression system: Escherichia coli BL21(DE3)