2v5e

X-ray diffraction
2.35Å resolution

The structure of the GDNF:Coreceptor complex: Insights into RET signalling and heparin binding.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GDNF family receptor alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.54 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q62997 (Residues: 150-349; Coverage: 45%)
Gene names: Gdnfra, Gfra1, Retl1, Trnr1
Sequence domains: GDNF/GAS1 domain
Glial cell line-derived neurotrophic factor Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P39905 (Residues: 111-211; Coverage: 53%)
Gene name: GDNF
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 58.9Å b: 75.7Å c: 105.5Å
α: 90° β: 91.9° γ: 90°
R-values:
R R work R free
0.187 0.184 0.237
Expression system: Spodoptera frugiperda