X-ray diffraction
1.9Å resolution

Crystal structure of the TetR-like transcriptional regulator LfrR from Mycobacterium smegmatis in complex with proflavine


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 20.7 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q58L87 (Residues: 1-189; Coverage: 100%)
Gene names: ERS451418_06031, lfrR
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 48.3Å b: 62.44Å c: 70.64Å
α: 63.88° β: 88.34° γ: 88.13°
R R work R free
0.198 0.196 0.234
Expression system: Escherichia coli BL21(DE3)