2v1s

X-ray diffraction
2.05Å resolution

CRYSTAL STRUCTURE OF RAT TOM20-ALDH PRESEQUENCE COMPLEX

Released:

Function and Biology Details

Reaction catalysed:
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
  • not assigned
Biological process:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-145970 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial import receptor subunit TOM20 homolog Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 73 amino acids
Theoretical weight: 8.02 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62760 (Residues: 59-126; Coverage: 47%)
Gene name: Tomm20
Sequence domains: MAS20 protein import receptor
Structure domains: Mitochondrial outer membrane translocase complex, subunit Tom20 domain
Aldehyde dehydrogenase, mitochondrial Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 13 amino acids
Theoretical weight: 1.39 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P11884 (Residues: 12-24; Coverage: 3%)
Gene name: Aldh2

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: C2
Unit cell:
a: 151.781Å b: 64.146Å c: 68.018Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.254 0.248 0.308
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided