› 2uxe

X-ray diffraction
2.9Å resolution

The structure of Vaccinia virus N1

Released:
DOI: 10.2210/pdb2uxe/pdb
PDB entry 2uxe coloured by chain, front view.
PDB entry 2uxe coloured by chain, side view.
PDB entry 2uxe coloured by chain, top view.
Source organism: Vaccinia virus
Primary publication:
Functional and structural studies of the vaccinia virus virulence factor N1 reveal a Bcl-2-like anti-apoptotic protein.
Cooray S, Bahar MW, Abrescia NGA, McVey CE, Bartlett NW, Chen RA, Stuart DI, Grimes JM, Smith GL
J Gen Virol 88 1656-1666 (2007)
PMID: 17485524

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative N1L Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 117 amino acids
Theoretical weight: 14.29 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q49PX0 (Residues: 1-117; Coverage: 100%)
Gene names: 44/47.1_rMVA_020, List025, N1L, VACAC2_037, VACCL3_037, VACV-DUKE-037, VACV_026, VACV_BRZ_SERRO2_026, VACV_CTGV_CM01_026, VACV_IOC_B141_048, VACV_IOC_B388_048, VACV_TT10_033, VACV_TT11_033, VACV_TT12_033, VACV_TT8_033, VACV_TT9_033, VAC_DPP10_037, VAC_DPP11_037, VAC_DPP12_037, VAC_DPP13_037, VAC_DPP15_037, VAC_DPP16_037, VAC_DPP17_037, VAC_DPP19_037, VAC_DPP20_037, VAC_DPP21_037, VAC_DPP25_037, VAC_DPP9_037, VAC_IHDW1_030, VAC_TKT3_017, VAC_TKT4_017, VAC_TP3_032, VAC_TP5_032, VACli_037, m8028L, mO028L, synVACV_037
Sequence domains: Poxvirus Bcl-2-like proteins
Structure domains: dsDNA poxvirus

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 71.682Å b: 108.999Å c: 70.088Å
α: 90° β: 110.58° γ: 90°
R-values:
R R work R free
0.237 0.237 0.244
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures.
Randall et al. (2008)
34 more

8 mentions without citation

How vaccinia virus has evolved to subvert the host immune response.
Bahar et al. (2011)
7 more