2roc

Solution NMR

Solution structure of Mcl-1 Complexed with Puma

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P97287 (Residues: 152-308; Coverage: 47%)
Gene name: Mcl1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Bcl-2-binding component 3 Chain: B
Molecule details ›
Chain: B
Length: 27 amino acids
Theoretical weight: 3.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ML1 (Residues: 130-155; Coverage: 14%)
Gene names: Bbc3, Puma

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DGSA-distance geometry simulated annealing
Expression system: Escherichia coli BL21(DE3)