2rf4

X-ray diffraction
3.1Å resolution

Crystal structure of the RNA Polymerase I subcomplex A14/43

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase I subunit RPA43 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 214 amino acids
Theoretical weight: 24.22 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46669 (Residues: 1-251; Coverage: 66%)
Gene names: O6271, RPA43, RRN12, YOR340C
Sequence domains:
Structure domains:
DNA-directed RNA polymerase I subunit RPA14 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 87 amino acids
Theoretical weight: 9.68 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50106 (Residues: 1-112; Coverage: 64%)
Gene names: RPA14, YD8358.11, YDR156W
Sequence domains: Yeast RNA polymerase I subunit RPA14
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 229.857Å b: 63.942Å c: 65.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.252 0.285
Expression system: Escherichia coli BL21(DE3)