PDBe 2ret

X-ray diffraction
2.21Å resolution

The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the Type 2 Secretion System of Vibrio vulnificus

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type II secretory pathway, pseudopilin EpsI Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 103 amino acids
Theoretical weight: 11.69 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MPZ1 (Residues: 58-143; Coverage: 60%)
Gene name: VV0219
Sequence domains: Type II secretion system (T2SS), protein I
Structure domains: GSPII I/J protein-like
Type II secretory pathway, component EpsJ Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 175 amino acids
Theoretical weight: 20.73 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MPZ0 (Residues: 40-214; Coverage: 75%)
Gene name: VV0220
Sequence domains: Type II secretion system (T2SS), protein J

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P1
Unit cell:
a: 63.288Å b: 79.145Å c: 82.079Å
α: 65° β: 69.19° γ: 69.49°
R-values:
R R work R free
0.188 0.185 0.245
Expression system: Escherichia coli BL21(DE3)