2r8r

X-ray diffraction
2.3Å resolution

Crystal structure of the N-terminal region (19..243) of sensor protein KdpD from Pseudomonas syringae pv. tomato str. DC3000

Released:
Entry authors: Nocek B, Mulligan R, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.38 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q883V3 (Residues: 19-243; Coverage: 25%)
Gene names: PSPTO_2245, kdpD
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 56.859Å b: 56.859Å c: 372.783Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.216
Expression system: Escherichia coli BL21(DE3)