PDBe 2qyn

X-ray diffraction
1.57Å resolution

Crystal structure of PDE4D2 in complex with inhibitor NPV

Released:

Function and Biology Details

Reaction catalysed:
Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-specific 3',5'-cyclic phosphodiesterase 4D Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08499 (Residues: 388-715; Coverage: 41%)
Gene names: DPDE3, PDE4D
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 58.1Å b: 80.626Å c: 163.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.248
Expression system: Escherichia coli BL21