› 2qwr

X-ray diffraction
2.21Å resolution

Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-394aa)R171C and bovine Auxilin (810-910aa)D876C in the AMPPNP intact form

Released:
DOI: 10.2210/pdb2qwr/pdb
PDB entry 2qwr coloured by chain, front view.
PDB entry 2qwr coloured by chain, side view.
PDB entry 2qwr coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis of J cochaperone binding and regulation of Hsp70.
Jiang J, Maes EG, Taylor AB, Wang L, Hinck AP, Lafer EM, Sousa R
Mol Cell 28 422-33 (2007)
PMID: 17996706

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock cognate 71 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 43.37 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19120 (Residues: 1-394; Coverage: 61%)
Gene names: HSC70, HSPA8
Structure domains:
Putative tyrosine-protein phosphatase auxilin Chain: B
Molecule details ›
Chain: B
Length: 92 amino acids
Theoretical weight: 10.75 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27974 (Residues: 813-904; Coverage: 10%)
Gene name: DNAJC6
Structure domains: DnaJ domain

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand ACY 1 x ACY
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 38.797Å b: 56.494Å c: 225.878Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.294
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

The HSP70 chaperone machinery: J proteins as drivers of functional specificity.
Kampinga et al. (2010)
34 more

2 mentions without citation

Structural basis of J cochaperone binding and regulation of Hsp70.
Jiang et al. (2007)
1 more