X-ray diffraction
2.4Å resolution

Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-386aa)R171C and bovine Auxilin (810-910aa)D876C in the ADP*Pi state

Source organism: Bos taurus

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock cognate 71 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 43.37 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
  • Canonical: P19120 (Residues: 1-394; Coverage: 61%)
Gene names: HSC70, HSPA8
Structure domains:
Putative tyrosine-protein phosphatase auxilin Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.94 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q27974 (Residues: 812-905; Coverage: 10%)
Gene name: DNAJC6
Structure domains: DnaJ domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 38.627Å b: 58.262Å c: 225.929Å
α: 90° β: 90° γ: 90°
R R work R free
0.212 0.208 0.279
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)