PDBe 2qpd

X-ray diffraction
3.25Å resolution

An unexpected outcome of surface-engineering an integral membrane protein: Improved crystallization of cytochrome ba3 oxidase from Thermus thermophilus

Released:
Source organism: Thermus thermophilus HB8

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome c oxidase subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 568 amino acids
Theoretical weight: 63.43 KDa
Source organism: Thermus thermophilus HB8
Expression system: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ79 (Residues: 2-562; Coverage: 100%)
Gene names: TTHA1135, cbaA
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Structure domains: Cytochrome C Oxidase, chain A
Cytochrome c oxidase subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 18.58 KDa
Source organism: Thermus thermophilus HB8
Expression system: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ80 (Residues: 1-168; Coverage: 100%)
Gene names: TTHA1134, cbaB, ctaC
Sequence domains:
Structure domains:
Cytochrome c oxidase polypeptide 2A Chain: C
Molecule details ›
Chain: C
Length: 34 amino acids
Theoretical weight: 3.77 KDa
Source organism: Thermus thermophilus HB8
Expression system: Thermus thermophilus HB8
UniProt:
  • Canonical: P82543 (Residues: 1-34; Coverage: 100%)
Gene names: TTHA1133, cbaD
Sequence domains: Cytochrome c oxidase subunit IIa family

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P41212
Unit cell:
a: 115.19Å b: 115.19Å c: 149.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.307 0.215 0.307
Expression system: Thermus thermophilus HB8