PDBe 2qn5

X-ray diffraction
3Å resolution

Crystal Structure and Functional Study of the Bowman-Birk Inhibitor from Rice Bran in Complex with Bovine Trypsin

Released:
Entry authors: Li HT, Lin YH, Guan HH, Hsieh YC, Wang AHJ, Chen CJ

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bowman-Birk type bran trypsin inhibitor Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 15.1 KDa
Source organism: Oryza sativa Japonica Group
UniProt:
  • Canonical: Q0JR25 (Residues: 119-251; Coverage: 57%)
Gene names: LOC_Os01g03360, Os01g0124400, Os01g0124401, P0037C04.19, P0044F08.1, RBBI3.3
Sequence domains: Bowman-Birk serine protease inhibitor family
Structure domains: Cysteine Protease (Bromelain) Inhibitor, subunit H
Cationic trypsin Chain: T
Molecule details ›
Chain: T
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P43212
Unit cell:
a: 69.653Å b: 69.653Å c: 158.169Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.256 0.322