PDBe 2qkl

X-ray diffraction
2.33Å resolution

The crystal structure of fission yeast mRNA decapping enzyme Dcp1-Dcp2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA-decapping enzyme subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 15 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P805 (Residues: 1-127; Coverage: 100%)
Gene names: SPBC3B9.21, dcp1
Sequence domains: Dcp1-like decapping family
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
mRNA decapping complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 11.3 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O13828 (Residues: 1-95; Coverage: 13%)
Gene names: SPAC19A8.12, dcp2
Sequence domains: Dcp2, box A domain
Structure domains: Arc Repressor Mutant, subunit A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 42.723Å b: 49.665Å c: 115.052Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.259
Expression system: Escherichia coli