PDBe 2qfa

X-ray diffraction
1.4Å resolution

Crystal structure of a Survivin-Borealin-INCENP core complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Baculoviral IAP repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15392 (Residues: 1-142; Coverage: 100%)
Gene names: API4, BIRC5, IAP4
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
Borealin Chain: B
Molecule details ›
Chain: B
Length: 62 amino acids
Theoretical weight: 7.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53HL2 (Residues: 15-76; Coverage: 22%)
Gene names: CDCA8, PESCRG3
Sequence domains: Nbl1 / Borealin N terminal
Inner centromere protein Chain: C
Molecule details ›
Chain: C
Length: 47 amino acids
Theoretical weight: 5.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NQS7 (Residues: 1-47; Coverage: 5%)
Gene name: INCENP
Sequence domains: Chromosome passenger complex (CPC) protein INCENP N terminal

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 42.467Å b: 78.793Å c: 100.017Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.202
Expression system: Escherichia coli