2qby

X-ray diffraction
3.35Å resolution

Crystal structure of a heterodimer of Cdc6/Orc1 initiators bound to origin DNA (from S. solfataricus)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
ORC1-type DNA replication protein 1 Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 43.92 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980N4 (Residues: 15-397; Coverage: 97%)
Gene names: SSO0257, cdc6-1
Sequence domains:
Structure domains:
ORC1-type DNA replication protein 3 Chain: B
Molecule details ›
Chain: B
Length: 384 amino acids
Theoretical weight: 43.47 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97WM8 (Residues: 14-394; Coverage: 97%)
Gene names: SSO2184, cdc6-3
Sequence domains:
Structure domains:
DNA (33-MER) Chain: C
Molecule details ›
Chain: C
Length: 33 nucleotides
Theoretical weight: 10.2 KDa
DNA (33-MER) Chain: D
Molecule details ›
Chain: D
Length: 33 nucleotides
Theoretical weight: 10.09 KDa

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 77.649Å b: 199.143Å c: 213.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.269
Expression system: Escherichia coli