› 2q79

X-ray diffraction
1.8Å resolution

Crystal Structure of single chain E2C from HPV16 with a 12aa linker for monomerization.

Released:
DOI: 10.2210/pdb2q79/pdb
PDB entry 2q79 coloured by chain, front view.
PDB entry 2q79 coloured by chain, side view.
PDB entry 2q79 coloured by chain, top view.
Source organism: Human papillomavirus type 16
Primary publication:
Increased stability and DNA site discrimination of "single chain" variants of the dimeric beta-barrel DNA binding domain of the human papillomavirus E2 transcriptional regulator.
Dellarole M, Sánchez IE, Freire E, de Prat-Gay G
Biochemistry 46 12441-50 (2007)
PMID: 17915949

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein E2 Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.16 KDa
Source organism: Human papillomavirus type 16
Expression system: Escherichia coli
UniProt:
  • Canonical: P03120 (Residues: 285-365; Coverage: 22%)
Gene name: E2
Sequence domains: E2 (early) protein, C terminal
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P3121
Unit cell:
a: 43.165Å b: 43.165Å c: 74.918Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.241
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A survey of the year 2007 literature on applications of isothermal titration calorimetry.
Bjelić et al. (2008)
7 other articles

2 mentions without citation

The critical protein interactions and structures that elicit growth deregulation in cancer and viral replication.
Ou et al. (2011)
1 more