2q0e

X-ray diffraction
2.1Å resolution

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound GTP

Released:
Source organism: Trypanosoma brucei
Primary publication:
Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferases.
Proc. Natl. Acad. Sci. U.S.A. 104 14634-9 (2007)
PMID: 17785418

Function and Biology Details

Reaction catalysed:
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.88 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q381M1 (Residues: 1-333; Coverage: 100%)
Gene names: TUT4, Tb11.01.7300
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 80.788Å b: 41.917Å c: 106.388Å
α: 90° β: 93.34° γ: 90°
R-values:
R R work R free
0.234 0.232 0.28
Expression system: Escherichia coli BL21(DE3)