2pzu

X-ray diffraction
2.1Å resolution

Crystal structure of Staphylococcal nuclease variant V66N/P117G/H124L/S128A at cryogenic temperature

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TNase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.78 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A5A523 (Residues: 69-215; Coverage: 89%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P41
Unit cell:
a: 48.22Å b: 48.22Å c: 62.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.211 0.265
Expression system: Escherichia coli