› 2pko

X-ray diffraction
1.8Å resolution

Crystal structure of yeast Urm1 at 1.8 A resolution

Released:
DOI: 10.2210/pdb2pko/pdb
PDB entry 2pko coloured by chain, front view.
PDB entry 2pko coloured by chain, side view.
PDB entry 2pko coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The sulfurtransferase activity of Uba4 presents a link between ubiquitin-like protein conjugation and activation of sulfur carrier proteins.
Schmitz J, Chowdhury MM, Hänzelmann P, Nimtz M, Lee EY, Schindelin H, Leimkühler S
Biochemistry 47 6479-89 (2008)
PMID: 18491921

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-related modifier 1 Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40554 (Residues: 1-99; Coverage: 100%)
Gene names: URM1, YIL008W
Sequence domains: Urm1 (Ubiquitin related modifier)
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21212
Unit cell:
a: 50.871Å b: 62.681Å c: 27.244Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Origin and function of ubiquitin-like proteins.
Hochstrasser M. (2009)
15 more

1 mention without citation

A conservation and rigidity based method for detecting critical protein residues.
Akbal-Delibas et al. (2013)