2pjp

X-ray diffraction
2.3Å resolution

Structure of the mRNA-binding domain of elongation factor SelB from E.coli in complex with SECIS RNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Selenocysteine-specific elongation factor Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 14.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P14081 (Residues: 487-607; Coverage: 20%)
Gene names: JW3563, b3590, fdhA, selB
Sequence domains: Elongation factor SelB, winged helix
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
SECIS RNA Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.39 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 103.498Å b: 56.515Å c: 48.414Å
α: 90° β: 95.6° γ: 90°
R-values:
R R work R free
0.204 0.2 0.251
Expression systems:
  • Escherichia coli BL21
  • Not provided