2pg1

X-ray diffraction
2.8Å resolution

Structural analysis of a cytoplasmic dynein Light Chain-Intermediate Chain complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only hexamer (preferred)
Non-polymer only dodecamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Dynein light chain 1, cytoplasmic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 91 amino acids
Theoretical weight: 10.64 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q24117 (Residues: 1-89; Coverage: 100%)
Gene names: CG6998, Cdlc1, ctp, ddlc1
Sequence domains: Dynein light chain type 1
Structure domains: Protein Inhibitor Of Neuronal Nitric Oxide Synthase;
Dynein light chain Tctex-type Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 111 amino acids
Theoretical weight: 12.63 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94524 (Residues: 1-111; Coverage: 100%)
Gene names: CG12363, Dlc90F, Tctex
Sequence domains: Tctex-1 family
Structure domains: Ribosomal protein S3 C-terminal domain
Cytoplasmic dynein 1 intermediate chain 2 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 33 amino acids
Theoretical weight: 3.68 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62871 (Residues: 132-164; Coverage: 5%)
Gene names: Dnci2, Dncic2, Dync1i2
Sequence domains: Cytoplasmic dynein 1 intermediate chain 2

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 116.969Å b: 119.87Å c: 211.702Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.253
Expression system: Escherichia coli