2pfp

X-ray diffraction
2.1Å resolution

DNA Polymerase lambda in complex with DNA and dCTP

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase lambda Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 37.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGP5 (Residues: 242-575; Coverage: 58%)
Gene name: POLL
Sequence domains:
Structure domains:
Template Chain: T
Molecule details ›
Chain: T
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
Primer Chain: P
Molecule details ›
Chain: P
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
Downstream Primer Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 55.893Å b: 62.853Å c: 140.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.245
Expression systems:
  • Escherichia coli
  • Not provided