2pfj

X-ray diffraction
3.1Å resolution

Crystal Structure of T7 Endo I resolvase in complex with a Holliday Junction

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Endonuclease I Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 17.15 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00641 (Residues: 1-149; Coverage: 100%)
Gene name: 3
Sequence domains: Phage endonuclease I
Structure domains: Restriction Endonuclease
27-MER Chain: Z
Molecule details ›
Chain: Z
Length: 29 nucleotides
Theoretical weight: 8.83 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
27-MER Chain: Y
Molecule details ›
Chain: Y
Length: 29 nucleotides
Theoretical weight: 8.97 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3221
Unit cell:
a: 92.977Å b: 92.977Å c: 124.265Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.268 0.267 0.298
Expression systems:
  • Escherichia coli
  • Not provided