X-ray diffraction
1.8Å resolution

Ternary complex of Profilin-Actin with the Last Poly-Pro of Human VASP


Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Profilin-1 Chain: P
Molecule details ›
Chain: P
Length: 139 amino acids
Theoretical weight: 14.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P07737 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic
Vasodilator-stimulated phosphoprotein Chain: V
Molecule details ›
Chain: V
Length: 16 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P50552 (Residues: 199-214; Coverage: 4%)
Gene name: VASP

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 37.467Å b: 75.46Å c: 180.741Å
α: 90° β: 90° γ: 90°
R R work R free
0.16 0.158 0.208
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided