PDBe 2pa8

X-ray diffraction
1.76Å resolution

X-Ray Crystal Structure of the D/L Subcomplex of the Sulfolobus Solfataricus RNA polymerase

Released:
Primary publication:
The X-ray crystal structure of RNA polymerase from Archaea.
Nature 451 851-4 (2008)
PMID: 18235446

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase subunit D Chain: D
Molecule details ›
Chain: D
Length: 265 amino acids
Theoretical weight: 30.33 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: P95989 (Residues: 1-265; Coverage: 100%)
Gene names: C04_051, SSO0071, rpoD
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit L Chain: L
Molecule details ›
Chain: L
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980K0 (Residues: 1-92; Coverage: 100%)
Gene names: SSO5577, rpoL
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
Structure domains: RNA polymerase, RBP11-like subunit

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I212121
Unit cell:
a: 69.703Å b: 93.346Å c: 128.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.247
Expression system: Escherichia coli