2p9i

X-ray diffraction
2.46Å resolution

Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only heptamer (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61157 (Residues: 1-418; Coverage: 100%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Structure domains:
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.82 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: A7MB62 (Residues: 1-394; Coverage: 100%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Structure domains:
Actin-related protein 2/3 complex subunit 1B Chain: C
Molecule details ›
Chain: C
Length: 372 amino acids
Theoretical weight: 41.02 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q58CQ2 (Residues: 1-372; Coverage: 100%)
Gene name: ARPC1B
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Actin-related protein 2/3 complex subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 300 amino acids
Theoretical weight: 34.4 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3MHR7 (Residues: 1-300; Coverage: 100%)
Gene name: ARPC2
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Structure domains: Yope Regulator; Chain: A,
Actin-related protein 2/3 complex subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 178 amino acids
Theoretical weight: 20.57 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3T035 (Residues: 1-178; Coverage: 100%)
Gene name: ARPC3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Structure domains: Actin-related protein 2/3 complex subunit 3
Actin-related protein 2/3 complex subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 168 amino acids
Theoretical weight: 19.7 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q148J6 (Residues: 1-168; Coverage: 100%)
Gene name: ARPC4
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)
Structure domains: Yope Regulator; Chain: A,
Actin-related protein 2/3 complex subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3SYX9 (Residues: 1-151; Coverage: 100%)
Gene name: ARPC5
Sequence domains: ARP2/3 complex 16 kDa subunit (p16-Arc)
Structure domains: Actin-related protein 2/3 complex subunit 5

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 110.61Å b: 128.184Å c: 198.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.261
Expression system: Not provided