› 2p80

Solution NMR

Solution structure of the complex between nitrite reductase and pseudoazurin from A. faecalis

Released:
DOI: 10.2210/pdb2p80/pdb
PDB entry 2p80 coloured by chain, ensemble of 20 models, front view.
PDB entry 2p80 coloured by chain, ensemble of 20 models, side view.
PDB entry 2p80 coloured by chain, ensemble of 20 models, top view.
Source organism: Alcaligenes faecalis
Entry authors: Vlasie MD, Ubbink M

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137609 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Copper-containing nitrite reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 341 amino acids
Theoretical weight: 36.88 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38501 (Residues: 40-376; Coverage: 98%)
Gene names: nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins
Pseudoazurin Chain: D
Molecule details ›
Chain: D
Length: 123 amino acids
Theoretical weight: 13.38 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04377 (Residues: 24-146; Coverage: 100%)
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 7 x CU
Ligand GD 9 x GD
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, rigid body molecular dynamics, restrained side-chains energy minimization
Expression system: Escherichia coli BL21(DE3)

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