2p7c

Solution NMR

Solution structure of the bacillus licheniformis BlaI monomeric form in complex with the blaP half-operator.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Penicillinase repressor Chain: B
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.51 KDa
Source organism: Bacillus licheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06555 (Residues: 1-82; Coverage: 64%)
Gene names: blaI, penI
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Strand 1 of Twelve base-pair DNA Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.67 KDa
Source organism: Bacillus licheniformis
Expression system: Not provided
Strand 2 of Twelve base-pair DNA Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: Bacillus licheniformis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing from randomized coordinates followed by restrained molecular dynamics.
Expression systems:
  • Escherichia coli
  • Not provided