2p5l

X-ray diffraction
2.85Å resolution

Crystal structure of a dimer of N-terminal domains of AhrC in complex with an 18bp DNA operator site

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Arginine repressor Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 64 amino acids
Theoretical weight: 7.43 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17893 (Residues: 1-64; Coverage: 43%)
Gene names: BSU24250, ahrC, argR
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*DCP*DAP*DTP*DGP*DAP*DAP*DTP*DAP*DAP*DAP*DAP*DAP*DTP*DTP*DCP*DAP*DAP*DG)-3') Chains: A, E
Molecule details ›
Chains: A, E
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
DNA (5'-D(*DCP*DTP*DTP*DGP*DAP*DAP*DTP*DTP*DTP*DTP*DTP*DAP*DTP*DTP*DCP*DAP*DTP*DG)-3') Chains: B, F
Molecule details ›
Chains: B, F
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: I212121
Unit cell:
a: 139.105Å b: 118.77Å c: 121.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided