2ote

X-ray diffraction
1.47Å resolution

Crystal structure of a monomeric cyan fluorescent protein in the photobleached state

Released:
Source organism: Clavularia sp.
Primary publication:
Structural basis for reversible photobleaching of a green fluorescent protein homologue.
Proc. Natl. Acad. Sci. U.S.A. 104 6672-7 (2007)
PMID: 17420458

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein cFP484 Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 24.8 KDa
Source organism: Clavularia sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y3 (Residues: 44-259; Coverage: 81%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 65.489Å b: 66.31Å c: 97.203Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.261
Expression system: Escherichia coli