2ol3

X-ray diffraction
2.9Å resolution

crystal structure of BM3.3 ScFV TCR in complex with PBM8-H-2KBM8 MHC class I molecule

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 15.57 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: Q5R1F1 (Residues: 23-116; Coverage: 98%)
Gene names: TRADV16D, TRAV16
Structure domains: Immunoglobulins
T-cell receptor beta chain V region E1 Chain: B
Molecule details ›
Chain: B
Length: 113 amino acids
Theoretical weight: 13.06 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P04214 (Residues: 22-136; Coverage: 97%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
H-2 class I histocompatibility antigen, K-B alpha chain Chain: H
Molecule details ›
Chain: H
Length: 279 amino acids
Theoretical weight: 32.08 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01901 (Residues: 22-300; Coverage: 80%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: L
Molecule details ›
Chain: L
Length: 100 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
RNA-binding protein 5 Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 1.04 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q91YE7 (Residues: 484-491; Coverage: 1%)
Gene names: Luca15, Rbm5

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P43212
Unit cell:
a: 102.523Å b: 102.523Å c: 198.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.284
Expression systems:
  • Mus musculus
  • Escherichia coli
  • Not provided