PDBe 2o93

X-ray diffraction
3.05Å resolution

Crystal structure of NFAT bound to the HIV-1 LTR tandem kappaB enhancer element

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear factor of activated T-cells, cytoplasmic 2 Chains: L, M, O
Molecule details ›
Chains: L, M, O
Length: 301 amino acids
Theoretical weight: 34.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13469 (Residues: 392-678; Coverage: 31%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains: Rel homology DNA-binding domain
Structure domains:
kappaB enhancer element, DNA 25-mer Chain: A
Molecule details ›
Chain: A
Length: 25 nucleotides
Theoretical weight: 7.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
kappaB enhancer element, DNA 25-mer Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 7.68 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 93.691Å b: 95.299Å c: 159.227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.283
Expression systems:
  • Escherichia coli
  • Not provided