PDBe 2o26

X-ray diffraction
2.5Å resolution

Structure of a class III RTK signaling assembly

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Soluble KIT ligand Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 145 amino acids
Theoretical weight: 16.44 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P20826 (Residues: 28-166; Coverage: 56%)
Gene names: Kitl, Kitlg, Mgf, Sl, Slf
Structure domains: Growth Hormone; Chain: A;
Mast/stem cell growth factor receptor Kit Chains: U, W, X, Y
Molecule details ›
Chains: U, W, X, Y
Length: 290 amino acids
Theoretical weight: 32.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P05532 (Residues: 25-314; Coverage: 30%)
Gene names: Kit, Sl
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 76.849Å b: 200.154Å c: 82.023Å
α: 90° β: 91.42° γ: 90°
R-values:
R R work R free
0.237 0.237 0.27
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21