2o19

X-ray diffraction
2.45Å resolution

Structure of E. coli topoisomersae III in complex with an 8-base single stranded oligonucleotide. Frozen in glycerol at pH 5.5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA topoisomerase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 659 amino acids
Theoretical weight: 74.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P14294 (Residues: 1-653; Coverage: 100%)
Gene names: JW1752, b1763, topB
Sequence domains:
Structure domains:
5'-D(*CP*GP*CP*AP*AP*CP*TP*T)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.39 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P43212
Unit cell:
a: 102.16Å b: 102.16Å c: 451.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.246
Expression systems:
  • Escherichia coli BL21
  • Not provided