PDBe 2nuu

X-ray diffraction
2.5Å resolution

Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero 12-mer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ammonia channel Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 415 amino acids
Theoretical weight: 43.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 25-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains
Nitrogen regulatory protein P-II 2 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 112 amino acids
Theoretical weight: 12.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC55 (Residues: 1-112; Coverage: 100%)
Gene names: JW0440, b0450, glnK, ybaI
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 99.691Å b: 107.865Å c: 280.162Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.171 0.249
Expression system: Escherichia coli