X-ray diffraction
1.65Å resolution

Accommodation of positively-charged residues in a hydrophobic specificity pocket: Crystal structures of SGPB in complex with OMTKY3 variants Lys18I and Arg18I

Entry authors: Bateman KS, Anderson S, Lu W, Qasim MA, Laskowski Jr M, James MNG

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with trypsin-like specificity.
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptogrisin-B Chain: E
Molecule details ›
Chain: E
Length: 185 amino acids
Theoretical weight: 18.65 KDa
Source organism: Streptomyces griseus
  • Canonical: P00777 (Residues: 115-299; Coverage: 71%)
Gene name: sprB
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Ovomucoid Chain: I
Molecule details ›
Chain: I
Length: 51 amino acids
Theoretical weight: 5.63 KDa
Source organism: Meleagris gallopavo
Expression system: Escherichia coli
  • Canonical: P68390 (Residues: 135-185; Coverage: 28%)
Sequence domains: Kazal-type serine protease inhibitor domain
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 45.307Å b: 54.803Å c: 45.486Å
α: 90° β: 119.02° γ: 90°
R R work R free
0.167 0.167 not available
Expression system: Escherichia coli