2nu0

X-ray diffraction
1.95Å resolution

Molecular structures of the complexes of SGPB with OMTKY3 aromatic P1 variants Trp18I, His18I, Phe18I, and Tyr18I

Released:
Entry authors: Bateman KS, Anderson S, Lu W, Qasim MA, Huang K, Laskowski Jr M, James MNG

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptogrisin-B Chain: E
Molecule details ›
Chain: E
Length: 185 amino acids
Theoretical weight: 18.65 KDa
Source organism: Streptomyces griseus
UniProt:
  • Canonical: P00777 (Residues: 115-299; Coverage: 71%)
Gene name: sprB
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Ovomucoid Chain: I
Molecule details ›
Chain: I
Length: 51 amino acids
Theoretical weight: 5.66 KDa
Source organism: Meleagris gallopavo
Expression system: Escherichia coli
UniProt:
  • Canonical: P68436 (Residues: 6-56; Coverage: 91%)
Sequence domains: Kazal-type serine protease inhibitor domain
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 45.35Å b: 54.52Å c: 45.65Å
α: 90° β: 119.2° γ: 90°
R-values:
R R work R free
0.145 0.145 not available
Expression system: Escherichia coli