PDBe 2ntc

X-ray diffraction
2.4Å resolution

Crystal Structure of sv40 large T antigen origin binding domain with DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Truncated large tumor antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.41 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: Q98ZP7 (Residues: 131-260)
21-nt PEN element of the SV40 DNA origin Chain: W
Molecule details ›
Chain: W
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Synthetic construct
Expression system: Not provided
21-nt PEN element of the SV40 DNA origin Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.47 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 64.489Å b: 68.341Å c: 97.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.205 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided