2nra

X-ray diffraction
3.1Å resolution

Crystal structure of Pi initiator protein in complex with iteron DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
PI protein Chain: C
Molecule details ›
Chain: C
Length: 276 amino acids
Theoretical weight: 31.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P03067 (Residues: 1-276; Coverage: 91%)
Gene name: pir
Sequence domains: Initiator Replication protein
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*GP*AP*AP*CP*AP*TP*GP*AP*GP*AP*GP*CP*TP*TP*AP*GP*TP*AP*CP*GP*TP*CP*T)-3' Chain: A
Molecule details ›
Chain: A
Length: 23 nucleotides
Theoretical weight: 7.1 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*GP*AP*CP*GP*TP*AP*CP*TP*AP*AP*GP*CP*TP*CP*TP*CP*AP*TP*GP*TP*TP*CP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.01 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, NSLS BEAMLINE X12B
Spacegroup: P6122
Unit cell:
a: 125.9Å b: 125.9Å c: 138.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.224 0.267
Expression systems:
  • Escherichia coli K-12
  • Not provided