› 2noo

X-ray diffraction
1.65Å resolution

Crystal Structure of Mutant NikA

Released:
DOI: 10.2210/pdb2noo/pdb
PDB entry 2noo coloured by chain, front view.
PDB entry 2noo coloured by chain, side view.
PDB entry 2noo coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Nickel binding to NikA: an additional binding site reconciles spectroscopy, calorimetry and crystallography.
Addy C, Ohara M, Kawai F, Kidera A, Ikeguchi M, Fuchigami S, Osawa M, Shimada I, Park SY, Tame JR, Heddle JG
Acta Crystallogr D Biol Crystallogr 63 221-9 (2007)
PMID: 17242515

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 502 amino acids
Theoretical weight: 56.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33590 (Residues: 23-524; Coverage: 100%)
Gene names: JW3441, b3476, nikA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand IOD 2 x IOD
Ligand NI 1 x NI
1 modified residue:
Ligand TYI 2 x TYI

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 43.47Å b: 92.248Å c: 116.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.21
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

A survey of the year 2007 literature on applications of isothermal titration calorimetry.
Bjelić et al. (2008)
8 other articles