2nmv

X-ray diffraction
2.95Å resolution

Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
UvrABC system protein B Chain: A
Molecule details ›
Chain: A
Length: 661 amino acids
Theoretical weight: 76.44 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37954 (Residues: 1-661; Coverage: 100%)
Gene names: BSU35170, dinA, uvr, uvrB
Sequence domains:
Structure domains:
UvrABC system protein B Chain: B
Molecule details ›
Chain: B
Length: 38 amino acids
Theoretical weight: 4.51 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37954 (Residues: 622-659; Coverage: 6%)
Gene names: BSU35170, dinA, uvr, uvrB
5'-D(P*TP*TP*TP*TP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.48 KDa

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 74.41Å b: 95.597Å c: 97.878Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.281
Expression system: Escherichia coli