› 2nmm

X-ray diffraction
2.7Å resolution

Crystal structure of human phosphohistidine phosphatase. Northeast Structural Genomics Consortium target HR1409

Released:
DOI: 10.2210/pdb2nmm/pdb
PDB entry 2nmm coloured by chain, front view.
PDB entry 2nmm coloured by chain, side view.
PDB entry 2nmm coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kuzin AP, Abashidze M, Forouhar F, Seetharaman J, Kent C, Fang Y, Cunningham K, Conover K, Ma LC, Xiao R, Acton T, Montelione G, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
A [protein]-N-phospho-L-histidine + H(2)O = a [protein]-L-histidine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-192574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14 kDa phosphohistidine phosphatase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 135 amino acids
Theoretical weight: 15.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRX4 (Residues: 1-125; Coverage: 100%)
Gene names: CGI-202, HSPC141, PHP14, PHPT1
Sequence domains: Janus/Ocnus family (Ocnus)
Structure domains: Janus/Ocnus

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 84.685Å b: 60.088Å c: 79.299Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.261
Expression system: Escherichia coli

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