PDBe 2mta

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF A TERNARY ELECTRON TRANSFER COMPLEX BETWEEN METHYLAMINE DEHYDROGENASE, AMICYANIN AND A C-TYPE CYTOCHROME

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Methylamine dehydrogenase heavy chain Chain: H
Molecule details ›
Chain: H
Length: 373 amino acids
Theoretical weight: 41.06 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P29894 (Residues: 45-417; Coverage: 96%)
Gene name: mauB
Sequence domains: Methylamine dehydrogenase heavy chain (MADH)
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Methylamine dehydrogenase light chain Chain: L
Molecule details ›
Chain: L
Length: 125 amino acids
Theoretical weight: 13.73 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P22619 (Residues: 64-188; Coverage: 95%)
Gene name: mauA
Sequence domains: Methylamine dehydrogenase, L chain
Structure domains: Methylamine/Aralkylamine dehydrogenase light chain
Amicyanin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.51 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Cytochrome c-L Chain: C
Molecule details ›
Chain: C
Length: 147 amino acids
Theoretical weight: 16.27 KDa
Source organism: Paracoccus denitrificans
Expression system: Not provided
UniProt:
  • Canonical: P29899 (Residues: 23-169; Coverage: 95%)
Gene name: moxG
Sequence domains: Cytochrome C oxidase, cbb3-type, subunit III
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 148.81Å b: 68.85Å c: 187.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 not available
Expression system: Not provided