2msr

Solution NMR

Solution structure of LEDGF/p75 IBD in complex with MLL1 peptide (140-160)

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MLL cleavage product N320 Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03164 (Residues: 140-160; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
PC4 and SFRS1-interacting protein Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75475 (Residues: 344-426; Coverage: 16%)
Gene names: DFS70, LEDGF, PSIP1, PSIP2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 86%
Refinement method: torsion angle dynamics, molecular dynamics
Chemical shifts: BMR25130  
Expression system: Escherichia coli